Can someone suggest an optimized protocol to extract both polar and lipid metabolites from a sponge?

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Abdul-Hamid Emwas

Dear Varun,

Thank you so much for your suggestions, This is great

best regards

Harald Hasler-Sheetal

Hi.

Chen et al 2014 applied a MeOH/MTBE/H20 extraction, to get an integrated metabolome/lipidome extration.

Chang Y, Zhang L, Lu X, Zhao C, Zhu Z, Wang F, Zhang J, Chen S, Zhao Y, Xu G. 2014. A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC–MS analysis. Metabolomics.

Dear Harald

Thank you so much for you suggestions, these are very relevant papers.

Best regards