I am seeking the correct way to measure zeta potential of bacteria. After harvesting and washing I suspended the pellet (which (100microl) was grown in 10 ml MRS broth) into 10 ml sugar solution; however then I could not get any zeta potential value. Now I am going to try to suspend them into different liquids i.e. 0,15M NaCl, Milli-Q ...of 2 ml, after harvesting and washing.
Do you have any suggestion on the quantity of bacteria to load the zeta potential cuvette and suspension liquid before to measure zeta pot?
Thank you
Cigdem
Hello Cigdem Yucel,
This is a challenging question for me for sure..!!! i have never tried a zetapotential of a bacterial suspension ever. But since the theory zeta potential is related to stability of the colloidal dispersion. But why are we studying bacterial suspension ?
mahesh
Hi,
There are some protocols in these papers that you may find helpful: http://pubs.acs.org/doi/pdfplus/10.1021/es303394w
http://pubs.acs.org/doi/full/10.1021/es1010253 (see the SI for this paper)
Hi Cigdem,
Below are two references that might help you:
http://pubs.acs.org/doi/abs/10.1021/jp003315%2B
http://www.jlr.org/content/40/8/1495.long
Good luck!
Julio
Hi Cigdem, it is probably easiest to measure in Milli-Q. From your description it sounds as if the zeta quality report in the Zetasizer indicates that the scattering intensity (ie the concentration of your bacteria) is too low. If you need to concentrate, you may want to consider the diffusion barrier method to reduce the sample amount. There is a paper on this method linked below. Good luck!
Conference Paper Challenges in the Measurement of Protein Electrophoretic Mobility
Dear Adeyemi, Julio and Ulf thank you for your contributions.
I have found out that to suspend into sugar solution was not the correct way. When I suspended into 0,15M NaCl or MilliQ, pH adjusted to desired value, I could get a value in between -3,5 and -4 mV. However it is still lower than the literature values ( around -10 and -15 mV).
Dear Ulf, I checked the diffusion barrier technique that you sent, it is done by Malvern. One I am using now is a different brand. I am trying to understand how I can proceed with that one.
Best
Cigdem
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