Why Cutting force coefficients or the edge coefficients will be negative in few cases of machining operation?

In mechanistic modeling of cutting forces in turning, identification of cutting force coefficients and edge coefficients through experiments are very much essential. these coefficients can be...

05 October 2019 3,864 3 View

What can control gene expression after irradiation (besides DNA methylation)?

I'm studying a gene whose expression is regulated by DNA methylation. Cancer cell lines that express this gene show strong hypomethylation, while cells lines that don't express it show...

19 March 2019 7,845 2 View

How to solve scattering problem in sidewall of any pattern devices by using maxwell formulation without simulations through FDTD and COMSOL?

Actually i am looking some theoretical model who can solve the semiconductor scattering issues.

22 August 2018 3,062 2 View

Is it possible to get yield more than 100% in a chemical reaction....?

Is it possible to get yield of a chemical product more than 100%...? If so, how one can defend this situation? I am working on Condensation reactions using organic solvent free synthesis. For some...

22 June 2018 2,453 21 View

Can I transfect the only 147nt ssOdna 5'-3' (only sense strand)as repair template for Knockin experiment?

27 August 2017 3,856 2 View

Hi - does anyone have any advice about retrieving PBMCs from their suspension in RNAlater?

The PBMCs were suspended in RNALter and then stored at -80oc. Now I want to retrieve the PBMCs and then extract the RNA (inc the microRNAs) - does anyone have advice about centrifuge speeds/...

19 June 2017 6,038 3 View

Molecular weight changes with GPI Anchors

I'm studying a GPI-anchored protein and all of the antibodies against this protein are ~5-7kD higher than the expected molecular weight (expected is ~18kD and most antibodies give ~25kD). I'm...

21 May 2017 1,323 5 View

Does anyone help me out to clone the Oligo sequence in Lenti CRISPRv2 backbone plasmid ?

18 May 2017 6,919 1 View

In ABAQUS: How to apply pressure loads at different locations?

In ABAQUS: How to apply pressure loads at different locations?

07 May 2017 1,676 4 View

Is there any way to in vitro methods for checking to anticancer properties of plant extracted substance without using animal cell line or MTT assay?

I am working on the phara quantification of plant substance, facing some experimental aspect , alike no availability of animal cell culture setup. So, anticancer assay aspect completely non in...

20 January 2017 8,296 5 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

Does euthanasia by CO2 inhalation cause NF-kB activation in mouse heart or body in general?

I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?

26 January 2021 4,096 2 View

How to measure the hardness for PLA & PHAs polymers?

Hello, I am a bit confused on whether to follow ASTM D785 (Standard Test Method for Rockwell Hardness of Plastics and Electrical Insulating Materials) or ASTM D2240 (Standard Test Method for...

21 January 2021 7,487 4 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View

What are technical problems working with HCT116 colon cancer cell line ? how is it maintained and cultured to study the anticancer effects of a drug?

Technical problems working with HCT116 compared with other cell line Caco-2 and HT29

07 January 2021 2,067 3 View

Is it possible to calculate the sample size of a clinical trial with the Cohen formula when there is no similar article?

We are trying to design a clinical trial on type 2 diabetes patients. The main data that we want to assess include FBS, 2hpp, HbA1c, insulin, and HOMA-IR. Also, we will assess the lipid profile...

21 December 2020 2,454 3 View

Blow extrusion of PLA with the HDPE non rotary die. Is it possible?

Hello, I managed to run a small blown extrusion line, head diameter 70mm / screw 45mm. The machine is to be used for extrusion of sleeves from 200 to 500mm wide. Unfortunately, I have a draw...

13 December 2020 2,824 5 View

Which are the best methods to graft the polymeric nano/micro particles with lipids?

Good references (with clearly written methods/protocols) are highly appreciated. Polymers could be any, e.g., PLA, PCL, PLGA, etc. Similarly, lipids could be DSPE, DMPE, DMPA, DPPE, DLPC, DOTAP,...

07 December 2020 5,085 4 View

How do you confirm your beta receptor antagonist is WORKING before you implement it in a big experiment?

I want to use a beta blocker in vivo but first I want to ensure the drug is working and for how long the effects last after the drug is withdrawn. I'm thinking I can administer the drug and then...

06 December 2020 5,179 1 View