I have transformed the leaves explants with agrobacterium tumefaciens to generate the stable transgenic plant lines. I have read many papers that checked the GUS expression after two days of co-cultivation for the localization of GUS. In the same way, is it possible to check the transient expression of gene of interest after two days of cocultivation?
Yes, you can immerse the transformed leaf in X-Gluc solution and can do the transient gene expression analysis.
Of course, you can do it. The expression will be determined in mesophyll indicating the agroinfiltration.
If it's GUS, check to see if your gene has an intron it, otherwise you can get GUS expression in Agro but it will just look superficially like blue samples.
Also, GUS staining kills your explants, so you'll need to pick a few to sacrifice.
I'm very interested also in this method. I have my own protocol, maybe it's helpful for you ;-)
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