i am trying to mimic the physiological micro-environment in microfluidics. i know the fact that the most abundant one is collagen.
can i use some other ECM proteins and add it with matrigel?
Matrigel already is abundant with Laminin. I do not see why someone would want to mix materigel with these laminin, but elastin and fibronectin might be an option.
Your precise answer really depends on your biological question. Matrigel is mainly used to mimic basement membrane of blood vessels. It is commonly referred to as tissue mimicking ECM especially in cancer research and the reason is that in cancer invasion field, all invading cancer cells have to somehow reach a blood vessel and pass through its basement membrane which is made from Fibronectin and laminin.
I agree with Kamyar, Matrigel contains laminin, (+ collagen IV, amongst others).
Plus it the most studied mixture of basement membrane proteins.
Google scholar results with Matrigel are ~130,000.
https://scholar.google.com/scholar?as_vis=1&q=matrigel&hl=en&as_sdt=1,23
Matrigel consists of collagen-1 aswell, shown on western blot. Why do you want to add additional proteins to matrigel, or do you produce 2gels?
Please see the paper through a link below, which shows the brief proteomics of matrigel showing all the proteins as you mentioned in your question. In supplementary figure, you can see the chart showing how much those proteins present in Matrigel. All the best!
http://advances.sciencemag.org/content/3/9/e1700764