I use high throughput sequencing to analyse the microbial communities in different soil samples. In addition, I woulk like to use the function indval (labdv package, R, Dufrêne and Legendre) to find the indicator species for each group. However, I do not know if it is a problem to apply this function on hellinger-transformed data, or if I have to use it on raw data. I tried both of them, and there are some differences.
Thank you