I'm trying to identify muscle reinnervation after a nerve injury. For that I want do inject some traccer on the muscle to identify which nerve is reponsible for the reinnervation. Which is the bast traccer, can BDA or DiI work in a short period of time?
I have never used BDA so I can't comment on it. DiI and DiO label lipid bilayers and thus are nonspecific. They will label muscle fibers as well as alpha-motoneurons. I think that if you want to study reinnervation, you will have to label the neuromuscular junction (NMJ) to examine its morphology. A fluorescently-labeled alpha-bungaratoxin is excellent for doing that. It is very specific. Perhaps you can do a double stain where you label both the NMJ and alpha-motoneurons.
Hello, Raquel,
For some time, veterinary surgeons have been looking into reinnervation of the cricoarytenoideus dorsalis muscle of the equine larynx utilizing neuromuscular pedicle graft techniques following natural and induced deinnervation and subsequent laryngeal dysfunction. I cannot cite specific articles; however, you might do a lit search in that area with authors John Stick, Johnathan Lumsden to see if they used specific immunohistochemical techniques which may add to your options. Apologies for not being able to be more specific at this juncture.
Hi Gordon, I've doner the muscle junction staining with alfa bungarotoxin, but i'm having trouble double labeling with some axon marker. Do you have any suggestions for that protocol?
No, Raquel, I have not done double labeling with an axon marker. I have always been more interested in the muscle side of the neuromuscular junction.
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