I have some extracts with sugars in low concentrations, so diluting isn't ideal, and there's about a 17 % of urea (from the solvent). Can the urea damage the column or affect the analysis?
Antonina Kokisheva
I suppose, if your mobile phase doesn t contain urea, you can try to inject sample, but your sugar must be highly soluble in the mobile phase, overwise inlet column frit becomes plugged.
If you have any doubts, read the instructions for the column or ask a representative of Bio-Rad
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