I am going to be staining tumor infiltrating lymphocytes from CT26 tumors, draining lymph node, and spleen. I want to look for two different TILs with different specificity. I have 2 tetramers specific for each, would it be possible to incubate the samples with both tetramers at the same time, then stain with the rest of antibodies? Or could stain with one, then the next, followed by the rest of my antibodies? Is there a chance that the two tetramers will interfere with each other, or increase non-specific binding?