Can I estimate lipid peroxidation and H202 in plant tissues after freezing or refregerating them?

More Jehan Salem's questions See All

My question is that how can we select the unique peptides ?

Hi all Scientists; I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis? I have done salivary gland proteomes for...

26 January 2021 7,488 3 View

How to prove the formation of a synthesis product using NMR ?

Hello, I am trying to identify the formation of a synthesis product. Knowing the precursors, I focused on the identification of the H-C long-range coupling between sites (a/b) and (a/c) using...

19 January 2021 8,438 5 View

Is there any default ''TopSpin'' [H-X] HSQC pulse program ?

I would like to conduct a [1H-13C]- HSQC (optimized for one bond H-C connection) experiment. What would be the best default topspin pulse program and parameters set to use ? Thank you.

14 December 2020 6,956 5 View

How can we quantitatively assess the value of an educational program (EP) ?

The question can be understood in terms of a secondary and auxiliary question, I.e. how we can say that EP1 has a greater intrinsic value as compared to EP2 or Vice versa ?

06 December 2020 916 5 View

Why does the command ''Topshim'' fails ?

Hello, I'am trying to run the 1H NMR spectrum of an ionic solution (Bromide Salt). the solution is too viscous so I had to randomly add a certain volume of deuterated chloroform, and then...

30 November 2020 8,569 1 View

How can I improve the Cronbach Alpha results without deleting too much items?

I am running pilot test and the Cronbach's alpha is 0.71 for all of the items. However, when I come to analyze every section of the questionnaire individually, In found that it is too low. And I...

10 October 2020 6,213 1 View

How to interpret DSC heat flow curves?

I'm running DSC for a mixture of 2 polymers and I need help to interpret the heat flow curves

13 August 2020 2,993 4 View

Someone from Alexandria University his name Mohammed Salem claimed three of my papers to him in research gate, how can this problem be resolved?

Mohammad Salem from the Alexandria University claimed the authorship these three papers: 1. First Report of Fusarium verticilloides causing Banana Fruit Rot in Jordan 2. First report of cucurbit...

26 July 2020 9,647 3 View

What is the best method to analyse survey data in auditing research ?

After the field data has been collated using Likert-style instrument e.g 'Strongly agree,Agree,Neutral to Strongly disagree, , what is the appropriate test statistic to use ? (i.e easy to...

18 May 2020 5,039 4 View

Can Neural Network predict an output value?

If I have a set of input data and a set of output data as shown in attached file, Can a Neural Network predict an output if the input data out of the given input data? Example: if the new...

11 June 2019 2,899 9 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

George Zervoudakis

Dear Jehan

I hope that the relevant discussion below will help you

https://www.researchgate.net/post/Can_I_measure_oxidative_stress_markers_in-80_frozen_tissue_from_last_year

Jehan Salem

Thank very much George Zervoudakis

Your reply was very useful for me.

Again thank you

Keli Souza da Silva

Dear Jehan,

you must freeze the seddlings in ultrafreezer (-80ºC).

Thank you Keli Souza da Silva