Good day fellow researchers.
I would like to know whether presence of fatty acid salts in ester products (containing free fatty acids and mono, di and tri esters) could have possibly hindered the derivatization of free fatty acids but not the esters component?
This is because I have conducted several replicates with excess derivatization agent, and prolonged the derivatization time, but at times, replicate can complete derivatize the free fatty acid while at other times, they failed. Theoretically, the fatty acid salts are not visible in the GC chromatogram due to non-volatile property correct?
Looking forward to more insights on the analysis of free fatty acid and fatty acid salts in ester products.
Thank you !
Fatty acid salts can hinder the derivatization of free fatty acids in GC analysis due to their ionic nature, causing inconsistent results. Fatty acid salts are non-volatile and usually do not appear in the GC chromatogram, unlike the esterified components.
Hello, if you perform methylation of fatty acids with sodium methylate in hexane than perform centrifugation, in upper layeryou will have only methylated fatty acids in hexane fraction and fatty acid salts will move to lower layer.
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