How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

What is the minimum number of cells required for fluorescence in situ hybridization?

I have to perform a FISH experiment with limited number of cells and I am trying to estimate the minimum number of cells required to do FISH. Cells are from mammalian cell line. I failed in doing...

12 January 2021 7,887 1 View

Can you give me some Information about the generation of ROS in marine surface water?

Reactive oxygen species is a very new term i think

23 December 2020 8,416 4 View

Can someone suggest me a wear detection method for a pipe which goes under intense heat and pressure at intervals?

I am looking for a wear detection method for a pipe that goes under intense heat and pressure for short instances at intervals. this process has an average cooldown time of 8 seconds between each...

08 October 2020 3,776 2 View

Single-cell cloning from SkBr3?

Did anyone tried single-cell cloning from SkBr3 cell line. I am failing in growing cells post single cell seeding in wells. Any help is appreciated

27 September 2020 8,762 4 View

What to do when RT PCR becomes positive again after 15-20 days of 1st sample positivity?

We are getting some patients who were initially positive of RT PCR for Covid. But in retesting after 15-20 days or even 20 days after those patients remain still positive. Is it for dead RNA...

17 August 2020 9,552 9 View

How to calculate % sequence identity using Clustal W?

I am calculating % epitope conservation for different mammalian antibodies against zebrafish. I have performed the Clustal W for antigenic peptide used in raising the antibodies against the...

05 August 2020 6,399 5 View

What is the Source of Harmonic Power ?

Consider a sinusoidal supply at fundamental frequency as shown, with a series inductive reactance leading to a bus. Let a diode rectifier load be connected to the bus. The switch Sw for resistive...

24 June 2020 8,175 27 View

What is the best book on Game theory from the perspective of Computer Science and Engineering?

I do not know much about game theory. I am finding a book where different protocols and algorithms of computer science have been analyzed using the concept of game theory.

17 May 2020 4,774 7 View

How can a PhD in interdisciplinary fields can benefit a researcher in job market?

I am a PhD scholar of Humanities and my research is broadly connected to Anthropological studies and framework. I have also published with an Anthropological journal of global repute and looking...

10 May 2020 4,202 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

How to assign two different material/section properties in Abaqus(UMAT)?

Hi everyone, I am conducting a non-linear multi-layer pavement structure analysis which is a stress-dependent. So far, I am able to define the nonlinearity for one soil layer for its resilient...

11 February 2021 9,607 3 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View