In this 2 journal 2 different process is written for Phosphomonoesterase activity,one is 425 nm another is 400 nm, which procedure is appropriate, kindly reply with the proper reference ?
Shrikanth VM, Janardhan B, More SS, Muddapur UM, Mirajkar KK. In vitro anti snake venom potential of Abutilon indicum Linn leaf extracts against Echis carinatus (Indian saw scaled viper). Journal of Pharmacognosy and Phytochemistry 2014; 3(1):111-117.
Janardhan B, Shrikanth VM, Mirajkar KK, More SS. In vitro screening and evaluation of antivenom phytochemicals from Azima tetracantha Lam. leaves against Bungarus caeruleus and Vipera russelli. Journal of Venomous Animals and Toxins including Tropical Diseases 2014, 20:12
Thats using pNPP as substrate right. The product yellow coloured nitro-phenolate shows maximum absorbance at 400 to 420nm. Its reported in many papers. See at which wavelength you get absorbance maximum and carry out the assay.
Many use this assay. You can find it in this publication. All the best.
Article Acid Phosphomonoesterase and Phytase Activities of Wheat (Tr...
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