one more thing, glyphosate by LC/MS will suffer the matrix suppression severely and you would need internal standard to correct for this. I am working on using a single column to monitor glyphosate, glufosinate, AMPA, forsetyl alumina, daminozide, maleic hydrazide, ethephon, paraquat, diquat, chlomequat, mepiquat, and ametrol. These are weed killer and plant growth regulator that are too polar to be retained on the C-18 column and cannot be partition by water/acetonitrile salting out. You need total extraction, filter, and shoot. There are many methods in the internet to cover these but I want to use only a single column to monitor these. May by need to run, one for positive and one for negative mode (10 min each) using a mixed-phase mode column. Anyone want to collaborate, please let me know.