This is the result of rice actin gene run for 25 cycles with 6 samples.
This may depend on your priming strategy for beta actin.
Beta actin has roughly 100 Pseudogenes in the genome. Some of them are actually expressed as parts in other mRNAs. There is a group of mRNAs that gets transported to the cell membrane because they have the ACTB pseudogene within their 3'UTR.
Therefore, I recommend that you check your primer (for example with the primer blast in Genome browser) for the regions in the genome they are binding to.
Sorry, Rice!
Than it is more complex. I am not a plant researcher. My knowledge is only derived from human genetics. The problem might be somehow similar to humans though, but not yet known in rice.
In humans we do not use ACTB that often anymore as a control gene in cDNA quantification. It has been a "painful" endeavor.
This site is helpful:
http://rice.plantbiology.msu.edu/analyses_search_blast.shtml
That looks familiar to me even though it is rice. Sequences of ACT1-ACT8 are everywhere in the rice genome.
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