I dont see many methods... Sap extraction with a Scholander pressure chamber is one of those. But be aware that if the overpressure is too high the exuded sap might be contaminated with symplasmic fluid (by reverse osmosis).
Thanks Hervé Cochard for your answer. But the problem is for the small leaves, but in case of broad leaves (eg Bean) we can extract by infiltration/pressure with Syringe method. I am really struggling to find out the convenient way for small and hard leaves
Dear Phillip Morris,
Thanks a lot for your suggested method. I think it will also work for rice. Can you please send me a link or a paper of you where I can find the detailed procedure.
Best regards
Ashraf
Hi Ashraf,
You can use a similar method that Phillip said also with Arabidopsis leaves, just infiltrating sterile miliQ water with a syringe (without needle) and then centrifuging the leaves (you will get around 10 ul of liquid from a 20 mg leaf). You must control that you are getting only apoplast, previously determining GADPDH activity in the same conditions.
Please find attached two other methods which could be useful.
All the best!
http://www.scigine.com/method.php?ID=193008
http://www.scigine.com/method.php?ID=278863
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