Can i use DTT during a Zn affinity chromatography?

I need to purify His6 tag containing protein and im using a Histrap that is been charged with Zn2+. Can i add DTT to my lysis buffer or the column will not tolerate it when i insert the lysate to...

12 October 2020 5,910 4 View

How can I solve a checkpoint file problem in Gromacs 2018.4?

Hello everyone, I am running a simulation for 500 ns but after 200 ns I get the following error: The input requested 100000000 steps, however the checkpoint file has already reached step...

16 January 2020 454 7 View

How to model Constant-Current Constant-Voltage Charging of Lithium-Ion Battery in Comsol?

I am trying to implement a constant-current constant-voltage charging strategy using the COMSOL standard lithium-ion battery model that comes with the chemical engineering module. The idea is to...

02 December 2019 9,563 3 View

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts)?

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts). Degassing takes place with 5 cycles of 1 minute degassing in vacuum and 1 minute...

18 November 2019 5,423 2 View

SEB or SEE for Jurkat and primary human/mice CD4+ T cell activation?

25 July 2017 338 7 View

How to check the observability of a LPV system?

Consider the discrete system x' = A(t)x + B(t)u y = C(t)x' + D(t)u The matrices A, B, C, D contain time-varying parameters (calculated by a second model) which are given in the initial state, but...

19 May 2017 2,813 1 View

How to detect low amounts of mRNA by qPCR?

I want to detect early expression of IL2 by qRT-PCR. I was not successful using random hexameric primers at the RT step and SYBR for detection. what can I do to improve my sensitivity? What...

01 February 2017 7,322 4 View

How do I calculate sensitivity of android acccelerometers?

Hello, I would like to make a sensitivity experiment on android built-in accelerometers (tri-axonal) an make a statistical analysis (correlation etc) in Matlab. My questions are: 1..What would be...

08 November 2016 9,127 1 View

Any suggestions for efficient Raji cell transfection?

Hi everyone. Has anyone worked with Raji cell transfection? I recently used AMAXA technology to transfect Raji cells with a CRISPR construct that resulted in a very poor efficiency (~1%). When I...

17 May 2016 5,476 4 View

Could Raman spectroscopy reveal the presence of Pt and Ru nanoparticles dispersed on a carbon powder?

09 November 2015 8,352 5 View

No title

02 March 2021 932 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View