Suggest the solvent to swell the gel and eluting solvent. Is it good enough to remove resinous and gummy materials from oleresins to isolate the compounds?
What's you compound soluble in?
The link below shows solvents that can be used. I've had good luck with water, methanol, dichloromethane, and methanol/dichloromethane mixtures.
https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1314735988470/litdoc56119097AD_20110830235244.pdf
What's you compound soluble in?
The link below shows solvents that can be used. I've had good luck with water, methanol, dichloromethane, and methanol/dichloromethane mixtures.
https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1314735988470/litdoc56119097AD_20110830235244.pdf
Dear Dr.Jack,
Mine is not a compound, but oleoresin. It is soluble in hexane, diethyl ether etc. This resin contain triterpenoids. We had isolated some of them by column chromatography.
But all fractions need to purify again and makes life difficult. I was thinking whether to use Sephadex LH-20 lipophilic-
to eliminate the resinous and gummy material by Lipophilic sepha dex LH -20
That may work...I'd consider running in hexane as you mentioned, or dichloromethane. No guarantees though....just need to run it on a small column and see if it works.
Thanks for the advise. I was thinking in the same way. We will do a pilot work see how it works. Thanks again for sharing thoughts.
regards
Hisham
- Badges
- Science topic