Can anyone provide me with working stripping protocol for PVDF membrane ?

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Christo Ananth

A PVDF membrane is highly recommended to minimize loss of sample protein. Note also that colorimetric/chromogenic detection reagents will leave a permanent visible stain on the membrane that can interfere with subsequent detection of targets of similar molecular weights. Chemiluminescent reagents such as ECL are recommended as they will not leave a stain and are more sensitive than colorimetric reagents.

And Dear Pranav Joshi Sir,Efficiency of stripping can be checked by incubating the membrane with chemiluminescent detection reagent. If stripping is judged to be satisfactory, rinse the membrane several times with buffer, then block before proceeding to the antibody incubation.

Pranav Joshi

Thanks Kevin for your suggestion.