How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Is a wall with constant pressure and constant inlet flow similar to an inlet boundary?

At an opening in turbulent flow, using wall boundary condition, with constant flow rate and constant pressure inlet, would be similar to an opening with inflow BC?

03 February 2021 2,390 5 View

How to amplify specific pcr product?

Hello I try to amplify gene of interest by RACE-PCR. For 5` RACE, I saw multiple bands after using specific nested primer. But The desired band was not seen on the gel. What is the reason? When I...

31 October 2020 7,734 6 View

What is the best force field for proteins with folded and disordered regions?

Hello I want to do MD simulation on a protein model to refine its structure. My protein structure have both folded and intrinsically disordered regions. What force field should i choose for this...

23 August 2020 9,381 5 View

What is the best force field for protein MD simulation?

Hello I try to do molecular dynamic simulation with GROMACS software and I work on an enzyme in a plant producing a medicinal secondary metabolite. When i was searching to find a appropriate force...

31 July 2020 171 5 View

How to avoid smear from PCR product on agarose gel?

Hello I used high fidelity enzyme in my PCR and I saw smear on agarose gel. I repeated the PCR with different amounts of cDNA. But I did`nt get a sharp band. what should i do to resolve my problem?

29 July 2020 3,483 10 View

Which one is more important in protein homology modeling ?

I try to make a model with MODELLER software and I decide to use multiple template modeling method. Two template structure I choose for my work, have similar Identity percentage (42.54% and...

03 May 2020 1,263 3 View

How can i detect small size PCR product from primer dimer?

Do you know any method to distinguish small target band of primer dimer? ( They have similar size.) I tried higher concentration of agarose gel and longer electrophoresis run time. but i didn`t...

22 December 2019 387 9 View

Is it possible to estimate the most recent common ancester using o & 1 data? If yes would you please introduce a software?

Dear professors Respectfully, i know that sequences can help us to reveal the most common ancestor in phylogenetic studies. However, in genetic diversity studies using IRAP, SSR, markers etc, i...

13 November 2019 9,305 6 View

Identify DNA bands transfer from gel to the membrane(southern blotting)?

Hello Is there a way to detect transferring of DNA bands from gel to membrane in southern blotting without staining? Thanks

10 November 2017 7,804 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

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22 February 2021 9,932 11 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View