Can someone guide me how to transport Live Ciliates samples in a transport medium?

01 February 2017 5,992 5 View

I've tried to recover DNA isolation through FastPrep Soil Kit but failed to recover it twice. Can anyone please guide me how to solve the problem?

First directly spun the sample at took the sediment from Glacier and proceed it according to FastPrep Soil Kit protocol. Secondly, i treated the sample with Guanidine thiocynate, Tris-HCl and 10%...

11 December 2016 3,128 4 View

Which Media can best isolate the Extreme Cold Adapted bacteria?

Cold adapted Bacteria Culturing Strategies, isolation through conventional microbial techniques

10 November 2016 5,571 12 View

How can we Lyophilize Snow samples?

What is the effective way to preserve the Microorganisms during the process? As it will reduce the quantity and will be much easier to transport the viable temperature sensitive...

10 November 2016 3,046 8 View

Can anyone please guide me how to prepare (1 ml of 4M Guanidine thiocynate in 0.1M Tris-HCl buffer pH 7.5)?

It is named Solution A, further added 150ul of 10% N-lauroyl sarcosine for water samples prior to DNA extraction.

01 January 1970 9,770 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View