Is it possible to estimate chlorophyll content by using titration method?
An evaluation of noninvasive methods to estimate foliar chlorophyll content; Andrew D. ichardson,
Shane P. Duigan,Graeme P. Berlyn; New Phytologist,
Volume 153, Issue 1.
Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non - destructive chlorophyll assessment in higher plant leaves; Anatoly A. Gitelson., Yuri Gritz., Mark N. Merzlyak. Journal of Plant Physiology Volume 16
Spectrophotometric Determination of Chlorophylls and Pheophytins in Plant Extracts. L. P. Vernon., Anal. Chem., 1960, 32 (9)
You should check;
Anatoly A. Gitelson, Yuri Gritz †, Mark N. Merzlyak, Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves, Journal of Plant Physiology, Volume 160, Issue 3, 2003, Pages 271-282
Leaf chlorophyll content and surface spectral reflectance of tree species along a terrain gradient in Taiwan’s Kenting National Park Jan-Chang CHEN, Botanical Studies (2007) 48: 71-77.
Simply write , protocol and procedure for estimation of chlorophyll . Then use google ,
or get book written by sadasivum and manikum , standard methods of plant biochemistry.
If I am not wrong it is like...take .5 gm leaves sample and add 10 ml DMSO and put it in oven(100 degree) for 3 hrs and take OD for chl a at 663, and for chl b at 645 nm.
Hi, this paper gives you both the destructive and non-destructive methods. Best iwshes.
https://www.researchgate.net/publication/225231248_Effect_of_fertilizer_application_on_photosynthesis_and_oil_yield_of_Jatropha_curcas_L?ev=prf_pub
Article Effect of fertiliser application on photosynthesis and oil y...
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