Isolated from estuarine area (10 ppt) by agar plating (f/2 medium). Black small colonies formed after 4 weeks. The size of cells varies from 8 to 20 micron. Cells are attached together and benthic in culture.
Dear Sandeep!
After your description and micrographs it seems to be coccal cyanobacterium Stanieria cf. sublitoralis (Lindstedt) Anagnostidis et Pantazidou 1991. It is a marine, epilithic cyanobacterium, which grows usually on limestone substrate, less comonly epiphytic or on calcareous shells of animals (also on non-calcareous coasts). Please check the book: Jiri KOMAREK & Konstantinos ANAGNOSTIDIS, 1998. Cyanoprokaryota, 1. Chroococcales. - In: H. Ettl, G. Gärtner, H. Heynig & D. Mollenhauer, Suesswasserflora von Mitteleuropa, 19/1, Gustav Fischer Verlag Jena, Stuttgart, Lübeck, Ulm, p. 403-404.
Best wishes
Bohuslav Uher
Estoy de acuerdo en que se trata de Chroococales sp ya que no le veo los cromoplastidios bien definidos por lo que puede ser una Cyanoprocariota.
Dear Sandeep,
I don't think it is a Chlorophycean alga since we cant see chloroplast in the cells. It could be a Cyanobacterium. My suggestion is that it could be a species of Aphanocapsa from Chroococcales.
Good luck!
If your algal culture is axenic, it is suggested to have DNA identification.
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