can anyone give me information about various selective media for production of biosurfactants? i already used Bushnell haas medium for production of Bio surfactants from Bacillus subtillis but results were not good as i expect.
Bushnell Haas medium is not designed for biosurfactant production, it is just a mineral salt medium used to isolated oil degrading bacteria. The key ingredients are sugar and oil source, some organisms like Pseudomonas doesn't need sugar and it can produce rhamnolipids directly from oil. See the attached article and try some thing similar. Also you need to optimize the fermentation conditions for your strains to get maximum yield.
Thank You Mr. Thavasi Renga Thavasi.
but in this paper author use peptone in production media, which also act as emulsifier. so results will be affected.
Dear Mr. Rakesh, you should find out in advance about your microbe characteristics. microbe-producing biosurfactant is specifics in its nature whether it produce biosurfactant constitutively or inducible/adaptive. Some microbe will produce biosurfactant in any growth medium while some other will only produce biosurfactant in the desired environment (extreme, lack of something, inducer substrate, so on). By knowing its characteristic, you will able to construct your own growth production (find the optimum fermentation conditions for your strains)
Hi, I agree with Mr. Qomarudin, first you need to find the optimum medium for your microorganism in order to reach the maximum growth. then you can add some oils such as olive oil ( 1% v/v) to induce production of biosurfactant. examine each medium with oil spreading method ( 5cc distilled water + 15 microliter crude oil + 15 microliter supernatant of your medium ) to find the best medium for biosurfactant production.
dear miss Farzaneh Fakharian. Thanks for reply. i will go for optimization process by various types of medium, but i want to know which components will be affect the results of screening of biosurfactants. for ex. if i add peptone it will show surfactant properties itself. which cannot give clear idea that biosurfactant was produced or not.....
For fast screening methods, you can consider the oil spreading technique as Ms Fakharian suggested, or just simply check the surface tension of the cell free broth or crude biosurfactant. One that give the lowest value compared with control medium should give clear idea whether biosurfactant was produced.
in this way you can extract and purify the biosurfactant so that you can find out whether it was biosurfactant or not. to extract the metabolite :
centrifuge the culture broth ( 7,000xg, 20 min, 4oC) inorder to remove the cells
adjust the pH of medium to 2.0 mL by adding 6 N HCl and kept at 4oC overnight to precipitate the biosurfactant
then centrifuge the supernatant at 10,000xg for 20 min
extract the mass at the bottom of tube 3 times with ethyl acetate
then spot it on the plate for TLC
Thanks to all of you... but seriously i don't find any satisfied answer. i know these all techniques. i just wanted to know a suitable media for production of surfactin.......
suitable media = optimum media?
each species have its own "suitable media" even the same genus of species. My suggestion is just follow other researchers method and medium that work with the same microbe that published their paper in any reputable journal.
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