Is there an open source tool the measures the GRAVY index of a peptide incorporating the PTMs and unusual amino acids (e.g. hydroxyproline)?

Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...

29 January 2021 8,300 4 View

What do you mean by 'excitation' of a plasma wave? What is the physical significance of this 'excitation'? Why is it necessary?

I have read numerous research papers on various mechanisms of excitation of surface plasma wave. But why do we need to excite a wave? Is it necessary? Can't the wave propagate without being excited?

25 January 2021 3,790 3 View

What is the difference between research summaries and abstracts? Can an abstract be contained in a research summary?

Some articles confuse the two subjects. I need to find out if they can be used interchangeably. If not, what is the difference between the two?

13 January 2021 847 3 View

How long does the peptide cleavage cocktail last before it gets expired?

The cleavage cocktail is composed of Phenol/thioanisole/water/TFA/triisopropylsilane. I only prepare 10 mL cocktail per peptide but can the activity of the cocktail prepared at 100 mL last for a week?

10 January 2021 7,424 3 View

How to present and discuss results, when methodology is not data-based?

Dear Everyone! I'd ask you opinion about a problem, which is returning in my "softer" research field. Time to time I work on research projects, which do not have available data and/or the data...

29 December 2020 7,637 6 View

Do anyone know a lab in Mexico where dielectric properties of solids and liquid can be measured?

I mean those kits that can be connected to vector network analyzers in order to find out permittivity and permeabilitty of solid and liquid from around 1 to 10 or 30 GHz

25 November 2020 852 1 View

How would you describe the most relevant research questions and directions in urban air pollution, if I'm specially interested in transportation?

I am relatively new to this field, my research interests are smart cities, shared mobility, transport service integration. We have data about CO, NO2, O3, PM10 from several cities. I would like to...

11 November 2020 9,099 4 View

How relevant is the Area under curve (AUC) in ROC being different from .50?

I analyze the psychometric qualities of a screening instrument to predict depression after stroke using ROC analysis. In this context, I read several papers which report an AUC without a p-value...

12 October 2020 6,557 4 View

How do you guys measure the rate of protein translation for a specific protein?

I am trying to think about a way to measure the protein translation rate for a single and specific protein that I am interested in. I've been thinking that Ribo-seq might be a good idea for this...

07 September 2020 9,054 3 View

Determination of Lipid A in Salmonella?

Im working with a S. typhimurium strain. Until now when i wanted to have a look at the amount of Lipid A present in the cells, i did silverstainings with proteinaseK treated whole cell samples....

26 August 2020 9,455 1 View

How can we draw the adsorption isotherms of Langmuir and Freundlich together with the experimental data in a single graph on Excel?

Thanks in advance

22 December 2020 7,853 4 View

Can I use gas concentration in the Langmuir equation?

Hi, there are questions about applying the Langmuir equation for gas adsorption. In one sentence, I wonder if it is okay to use C as the amount of gas supplied in the Langmuir equation. q=q_sat *...

06 September 2020 5,319 12 View

Is it possible to reuse Toluene after PAH Extraction with soxhlet?

In my lab, we are using Toluene for extraction of PAH from biochar (byproduct of pyrolysis). There are numerous samples to measure. Therefore, to make it economic can we distill toluene from the...

03 September 2020 6,111 5 View

About use of Fluoranthene as standard in HPLC?

Dear all; I want to work on the ecotoxicological effect of PAH (fluoranthene) on different organism species.... But I can not find its analytical standard in my country, Can I use Flournthene 98%...

05 July 2020 2,736 7 View

How to calculate Qmax value from Langmuir Equation?

I am doing dye removal experiment and need to calculate Qmax value from Langmuir Equation, Kindly help me with example .

08 June 2020 8,909 1 View

Do plant extracts contain essential oil compounds?

So I understand that essential oils are formed by steam distillation, and contain volatile, usually aromatic, compounds. Extracts are formed by immersion of plant matter in a solvent, and are...

12 May 2020 2,119 5 View

How can I make 3D model of a mutant tetramer enzyme(bearing compound heterozygous mutations)?

Hi I want to make 3D model of PAH enzyme(a tetramer E) to assess some compound heterozygote effects. Do you know any site to let me modify each subunit with mutation of interest?

22 March 2020 7,925 4 View

How can I calculate the distribution coefficient Ke value for Van't Hoff plot?

09 March 2020 3,002 5 View

How to calculate fix coefficient in non linear langmuir isotherm?

How to calculate fix coefficient in non linear langmuir isotherm?

09 February 2020 5,571 4 View

How to disperse polystyrene nanospheres in water surface for Langmuir Blodgett?

I've been testing several aproaches to getting a stable close-packed array of 518 and 617 nm nanospheres at the milipor subphase surface, for a future Langmuir Blodgett implementation. From what...

06 February 2020 8,391 1 View