How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

DOes someone know what is the best basis set and method for calculating excited state of ion CH5+ ??

I've been trying to do my calculations related to optimization of excited state of ion CH5+ using method TD-DFT with M06-2X and aug-cc--pVTZ. My input file is as shown...

03 January 2021 2,148 0 View

How can we choose the right method for Gaussian calculations?

I'm not working on biomolecules, I work on Organic, Organometallic, and sometimes inorganic structures. Do you know a simple way to apply the right calculation method? Is there any article or...

10 December 2020 3,396 5 View

Do Radial Basis Function Networks trained through Gradient Descent benefit from Logistic Regression?

When training a Radial Basis Function Classifier for classification problems, we generally train the model by making use of the pseudo-inverse of the design matrix, minimizing the MSE error...

09 December 2020 2,190 1 View

How to optimize the supercell (4x2) of rutile crystal structure TiO2 (110) by Gaussian 16?

I am doing structural optimization of the slab TiO2 (110) - (4x2 supercell) using Gaussian 16 (with PBC appproach) but because the structure is too large (96 atoms/supercell) the input files I...

28 November 2020 4,144 3 View

How to calculate Basis Set Superposition Error (BSSE) for interaction energy in SIESTA 4.0.1 code?

Hello, I'm calculating the interaction energy between molecule and graphene sheet using Eq. (E_i = (E_graphene + E_molecule) - (E_graphene+molecule). I have employed a numerical atomic orbital...

10 November 2020 8,467 2 View

What do i do when Gaussian 16 shows error during frequency calculation for lanthanides using mixed basis set?

I am trying to do frequency calculation after optimization calculations using mixed basis set for lanthanides SDD and 6-311++G(d,p) for other atoms with %mem=2GB. The error shows: Not enough...

21 October 2020 9,578 3 View

Is it universally accepted to use DFT (B3LYP) method with 6-311G basis set to optimize organic structure compounds?

I'm new in computational chemistry. I'm doing molecular docking on organic compounds towards enzyme. basically, I'm using B3LYP/6-311G to optimize all the ligand structure. Is it generally...

11 October 2020 3,273 13 View

Hot to convert s-1 to cm3 molecule-1 s-1 in kinetics?

Hi all, I have a conversion problem with rate constant calculations. My start point is the isoprene molecule with a chlorine atom bond to C1. I want to obtain the rate constant of: isoprene +...

27 September 2020 3,192 3 View

Should i have to change the structure of my transition state, optimized on lower basis set to run on higher basis set?

I have performed a transition state calculation. I am successful in optimizing it in gaussian at 6-31G(d,p). i tried to optimize it at 6-31+G(d,p) but it failed. what do you suggest should be...

22 September 2020 8,198 4 View