Does the material of the 96-well plate (PVC or Polystyrene) make a difference on the attachment of the biofilm to the surface? I am able to grow a mature Mycobacterium biofilm on the air-media interface, just need a better way of quantifying, as most of the bacteria is washed away when rinsed with saline. Plate counts works fine, but need a more high throughput method. Also, what stain will work the best to use for mycobacterial EPS?
Thank you for the reply. Much appreciated. I'll have a look at the different materials and will run some prep experiments to see what will work.
I will start by using a M. smegmatis strain that produces a mature biofilm in 3-4 days. I just want to quantify the amount of biofilm without staining the bacteria, if that is possible?
CV may not stain mycobacteria well. In that case, you may need to disperse biofilm and then do a CFU count.
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