What is the principle and application of this technique
I have found that http://www.protocol-online.org/ is generally a good resource for basic techniques and variations (as well as troubleshooting).
5 years of hearing professors at uni saying all the time that wikipedia is NOT a trustable source of information to see an experienced researcher posting a wikipedia link! tututut... ;)
@Gael: I know I use it too when I need a really general reminders on something. I was just kidding :P
Thankx friends for your reply i will surely look at these links
http://delangelab.rockefeller.edu/protocols_files/WesternBlot.pdf
http://www.ppsk.usm.my/lecturers/mravi/PDF_FIles/Westerb_Blot.pdf
http://www.piercenet.com/browse.cfm?fldID=8259A7B6-7DA6-41CF-9D55-AA6C14F31193
http://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf
Should we try to get a Western Blot Topic started? There is already one created!
http://www.researchgate.net/topic/Western_blot/
Who is in? ;)
Suggest some books to understand the interdisciplinary sciences involved in various techniques and biological computational tools.
03 April 2015 6,425 7 View
What is its role in human body
08 September 2011 6,669 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
23 February 2021 8,155 5 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...
16 February 2021 2,377 7 View
I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...
09 February 2021 5,155 5 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
04 February 2021 4,482 4 View
Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...
01 February 2021 780 3 View
I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...
17 January 2021 5,099 4 View
Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...
14 January 2021 4,383 8 View
Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...
05 January 2021 230 3 View