Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Requesting protocols (96 well plate) for superoxide radical scavenging activity and hydroxyl radical scavenging activity?

Hello everyone, I am currently working on the natural colour pigment, betalains. I am looking for 96- well plate adopted assay protocols for measuring superoxide and hydroxyl radical scavenging...

03 January 2021 2,414 2 View

Any good reference that links economic/industrial policy to changed agent behavior?

Usually industrial policy is interpreted as changing economic structure from in favor of more productive/advanced economic activities. But would like to read something that discusses the...

31 December 2020 3,199 3 View

How to simulate the magnetohydrodynamics (MHD) phenomena in COMSOL? What is the best way to learn how to use COMSOL?

I would like to simulate the Lorentz Force resulting from the magnetohydrodynamics (MHD) phenomenon in an ionic solution.

19 December 2020 8,278 9 View

RNA-Seq for the analysis of ribosomal RNA (rRNA) processing sites?

Greetings! I want to study ribosomal RNA expression and potential processing sites in bacteria. To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA...

02 December 2020 1,310 2 View

Problem executing MrBayes. Help?

Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...

18 November 2020 3,867 3 View

Has anybody seen research on Generative Adversarial Networks not image / video / autoencoder based?

Searching for Generative Adversarial Networks applications.

09 October 2020 2,316 2 View

Where could I find a list of the main journals for free-to-publish in marine ecology and marine biology fields?

I am trying to publish a couple of articles in order to improve my scientist CV...

23 August 2020 4,264 16 View

Does anyone have a protocol about growing 3D culture in matrigel in order to extract protein for Western Blot assay?

Hello. I'd like to perform a Western Blont with samples obtained from 3D culture in matrigel. I've done experiments with 3D cultures in 96 well plates in order to test drug activity, but now I'm...

27 July 2020 1,421 4 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Do you need permission to use a middle range theory in your dissertation?

I am using a figure of a middle range nursing theory, do I need permission to use?

03 February 2021 2,210 9 View

Any one can tell me primer sequences used for q PCR to quantify MERS-CoV related coronavirus?

MERS-CoV primers

31 December 2020 2,389 2 View

How do you decide if an assessment tool or questionnaire is not culturally appropriate ?

I have a validated tool in English ( the supportive care needs survey-short form 36) and I want to know if I can use this tool in Arabic population or should I develop a new questionnaire the...

12 December 2020 3,073 2 View

Voice recognition for alQuran Arabic?

How can we compare two audio files, or voice recorder files, according to Al-Quran. Al-Quran has special pronunciation compared to Arabic pronunciation. Is it possible to do the comparison between...

25 November 2020 3,657 7 View

Insect Lipase Activity?

Hi everyone! My name is Nacho. I am trying to assay lipase activity from insect midgut supernatant, and I have some problems and some doubts about it. I do not have the pH-stat. Thus, the...

22 November 2020 5,595 3 View

Add text at the beginning of a specific reference in Latex?

Hey researchers! The journal I'm submitting to wants datasets to be listed in the reference as [dataset] Authors; Year; Dataset title; Data repository ; Version; Persistent identifier (e.g....

18 November 2020 2,681 5 View

Do you need participants for an online study in Hebrew?

Dear Israeli students and researchers, You are invited to post your studies on the subreddit for surveys/studies in Hebrew (for free): https://www.reddit.com/r/Surveys_in_Hebrew/ Hopefully this...

12 November 2020 292 3 View