To understand the practical risk, cost benefit analysis and suitability to environmental remediation process.
First, in order to give you a reliable response to your question, you need to describe in more detail what YOUR "bioencapsulated" microbes are: lyophilized cells trapped in a (dry) gel which are being added to a wastewater (treatment plant)? If this is the case, in a longterm, some of these bugs may be retained in biofilms, and most washed out of the system, but probably do some "work" in the effluent. And it depends on the nature of xenobiotics (class of compound, degree of lipophylicity, and much more parameters.
Thank you mam,if lyophilized cells trapped gel used in WWT plant and most of the cells washedout happened, then the treatment efficiency was decreased, so what are the vital measures must one should follow to prevent the cells from washout and to retain it in the matrix till the end of the treatment process if i suppose to use organophosphate pesticides. thank you very much for your kind response and to share your valuable time.
Well, I assume that you intend to run a continuous process, as it is being found in wastewater treatment plants (WWTP). You may hope that these additional bacterial cells, upon revival in the lab before adding them to the process, settle on solid surfaces and form a catalytically active biofilm, or integrate into an already existing one. However, the solid surfaces found in real-life WWTPs are generally not sufficient for treating high loads or high volumes. And there is an additional problem: since most of the (toxic) xenobiotics are lipophilic they are mostly bound to particulate material in the wastewater stream, which means that they are not very much soluble and, therefore, little bioavailable. Unfortunately, this means that bioaugmentation has little positive effect if you do not recirculate at least 10 or 20% of the wastewater from the end of the system into the entrance.
Thank you Dr. Regina-MW mam, thank you very much for you kind response and sharing your opinion to me. Mam, the fed-batch process using biocatalytic bioencapsulated cells for the WWTP is effective for xenobiotic removal ...
For sure a (fed) batch process will work, but how do you retain the entrapped cells?
Thank you mam, if you come across any idea relevant to retain the entrapped cells kindly suggest me mam.
The only way is to have them introduced in a biofilm. For good efficiency you need a very large "catalytic" surface area!
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