01 January 2020 3 6K Report

Does anyone have/know any data on how the volume of recovery media affects things for electroporation of bacteria?

I see large ranges. For electroporating, eg, 200 uL concentrated bacteria, sometimes it's 1 mL (5 volumes), sometimes it's 10 mL (50 volumes). And some protocols hint that it matters a lot due to something about osmolarity. Eg, one protocol mentions recovery *must* be 10 volumes for "osmotic" reasons.

I'm also hoping to hear anything about duration of recovery too. Specifically for bacteria that have doubling times greater than 1 hr. I know for ecoli it's always 1 hr recovery.

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