Dear network

we are in the process of automating truseq library prep on biomek 4000. as such, the robot does what we ask it to do - transferring right volumes to righth places.

when comparing hand and robot method the libraries are widening from 200-500bp in the manual method to 200-1000bp on the robot.

anyone who has seen anything similar when automating? anyone who can comment on why and more importantly on how to fix it? increasing beads-to-DNA? increasing incubation time, washing - what would be the best way to go?

it seems to affect our otherwise robust CNV calls if we sequence these libraries so it needs to be fixed.