Anyone knows how to extract RNA from samples with high starch content (wheat) by using RNeasy Plant Minikit?

I am using 74904 to extract RNA from wheat seed and I have tried both of the lysis buffer. As it is mentioned it protocol, buffer RLT is the lysis buffer of choice but it may cause...

14 October 2020 9,409 7 View

How long does it take for MCF-7 cancer cells to adhere to the microfluidic surface? Is 24 hours enough?

Hello everyone. We have cultured MCF-7 cancer cells in microfluidics several times and incubated them for 24 hours, but as the nanomaterial passed through the cells, the cells moved and no cell...

05 September 2020 7,292 3 View

How can I make DMEM F12 with 30mM glucose concentration for induction of stress oxidative in HUVEC cells?

Hi dear researchers, DMEM F12 glucose concentration is 17.5mM, I added D_glucose in basal cell culture until reached 30mM, but HUVEC cells had better grown than control group after 48 hours. I...

06 April 2018 8,650 1 View

Clustering network vehiculer in ns2?

I need to compare my work with the Reservation-Based (RB)code that described in the "Offloading Floating Car data" article. Can anyone give me the its code? I work with ns2 simulator

06 September 2017 3,631 1 View

What is the longest lifetime reported for electron and holes in perovskite solar cells to date?

When these materials get excited with light, electron and holes are generated but it is important for them to hang arround for a while before they recombine so that more photocurrent will be...

26 August 2017 9,161 11 View

What is the density of poly vinyl alcohol (SB220)?

Density of PVA (SB220)

06 February 2017 8,921 5 View

Why the type of organic solvent can affect particle size of nanoparticles in film hydration method?

For example dichloromethane and chloroform

11 November 2016 1,428 1 View

How can i detect fire using SAR (RADARSAT-2) backscatter and optical data?

I would detect fire, insect and deforestation (logging and building) disturbance using SAR backscatter and Optical data for Hyrcnian forest in the Iran.

02 October 2016 2,129 4 View

How to prevent/reduce hysteresis effect in dye sensitized solar cells (DSSCs) / perovskite solar cells?

I have read that one of the major challenges in solar cells applications is reducing Hysteresis. First off, can anyone give an explanation of this phenomenon? Secondly, how to face this...

27 July 2016 8,620 5 View

Can we study Pests and dieback using L-band PALSAR data?

12 July 2016 6,369 2 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

What will happen (quality or quantity wise) if we transplant 'Aerobic Rice' in puddled condition?

I want to know about the consequences of aerobic rice (both quality and quantity-wise) when transplanted or sown in puddled condition?

08 February 2021 2,343 6 View

Does diseases like fungal,bacterial etc of rice plant is different from nutrient deficiency diseases?how deep learning is used except classification?

can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...

07 February 2021 470 2 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View