Methodology for Ni-NTA based affinity purification of His-tagged Beta lactamase?
I couldn't find one. This might be such an example:
Article What Makes a Protein Fold Amenable to Functional Innovation?...
I saw examples where the gene was cloned into a vector containing the His-tag N-terminal to the periplasmic export signal sequence, but the His tag would therefore not appear in the processed protein, and conventional chromatography was used for the purification.
Some journals claim that they will not accept kinetic data from enzymes containing tags and fusions, so there could be an issue with publication if you don't use the protein with the native sequence.
Article Characterization of a novel D-lyxose isomerase from Thermofl...
Dear follow this article he used HIS-Tag purification method with Ni-Affinity column from E.coli.
Thank you Waheed. But I needed the His-tag purification of TEM-1 Beta lactamase
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