Nitrogen is added to the nutrient medium with NO3- and NH4+ because the pH is between 5.4-5.8 which increases the ammonium ion uptake  to the plant tissue, resulting in a pH reduction of 4.2-4.6 which inhibits the ammonium ion uptake, which causes nitrate ions To the vegetative tissue, causing pH to rise again, etc. The reason for avoiding the addition of ammonium ions alone without nitrate ions as a source of nitrogen in the food medium is because its effect is called in the high concentration of it first and the difficulty of pH control.

Our lab is also facing the same problem now are using readymade MS medium which you can buy from any chemical company. We are also trying to find any other option but still, we could not find any.

Dear Doctor

Go To

Supplementation of Urea as the Nitrate Source of MS Medium and High-frequency Regeneration of Plantlets of Musa paradisiaca cv. Malnad Rasbale S. Ullas Prasanna, S. Ravi Kumar, Sachin S. Nayaka, S. Ajith, V. Krishna

Downloaded from https://cabidigitallibrary.org by 5.62.145.96, on 12/19/24. Subject to the CABI Digital Library Terms & Conditions, available at https://cabidigitallibrary.org/terms-and-conditions

[ABSTRACT Background: A consistent practice for mass multiplication of banana cv. Malnad Rasbale has been accomplished, which is an endemic cultivar of Western Ghats of Karnataka, India. Methods: Decrease in microbial contagion have been managed by dealing with outward sterilization agents ethanol, sodium hypochlorite, mercuric chloride solution, antibiotic solutions containing cefotaxime and gentamicin. MS media augmented with 350 mg/l urea as the nitrogen source by substituting KNO3 and NH4NO3 which is commonly used in terroristic activities. Organogenic media was supplemented with a range of 2-6 mg/l BAP and 0.3-2.7 mg/l TDZ. Result: The addition of urea substituting KNO3 and NH4NO3 gave the eminent grades in overall initiation, shoot multiplication and development and also in the rooting phase. Supplementation for shoot development with BAP (4 mg/l), TDZ (1.8 mg/l) and with 350 mg/l urea showed enthusiastic progress in shoot elongation with specific concentrations. Plants established under in vitro circumstances were reassigned to the greenhouse and after the progress, they were promoted to field conditions.

CONCLUSION We hereby conclude that in our present investigation culture establishment of Musa paradisica cv. Malnad Rasbale was successful. This study transparently shows us the synergistic effect of urea supplemented media replacing potassium nitrate (KNO3 ) and ammonium nitrate (NH4NO3 ) as macronutrient basis gave the best results in the stages of initiation, multiplication, shoot development and root development. So urea can also be used as a substitute for nitrates source in in vitro conditions. The outcome of this investigation can be applicable for micropropagation and mass multiplication of endemic species of banana Musa paradisica cv. Malnad Rasbale and field evaluation studies can be carried out further.]