What might trigger weaker bands in gel electrophoresis?

I have been getting increasingly weak gel electrophoresis bands over the past week following PCR. What might be the culprit? I added my red mix, primers, and H20 in the correct amounts. So is...

07 August 2017 7,080 9 View

In your opinion... are scientific PhD's too long or too short?

For the average expected coursework + research load

07 August 2017 6,146 0 View

Do professional schools in the US overstate their students' post grad employment salaries/ potential? If so, how?

07 August 2017 9,758 0 View

Will scientific academia ever be a dying field? If so, why? If you take a stance, please support your answer!

Feel free to have fun with this philosophical science/ biology/ chemistry/ physics/ etc question. I am hoping to get deep into it! I hope that together, we can hash out some of the key features...

07 August 2017 7,450 1 View

"Thought Probe" What are some of the most interesting things/ concepts/ events you've come across in Political Science and why?

Let your heart out

07 August 2017 6,713 0 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Hi, can a variable in a questionnaire be measured with a series of questions or it has to one question for one variable?

I am doing research on the impact of competitive bidding on project success and would like to use SEM. Project success shall be: cost overage, completion time, quality of works and variation...

13 February 2021 4,413 6 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

RWP1 growing in RPMI medium?

Hello everyone, Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details...

08 February 2021 6,455 1 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View