26 June 2013 4 7K Report

I would like to have your point of view.

More Yanick Simon's questions See All
Is there an open source tool the measures the GRAVY index of a peptide incorporating the PTMs and unusual amino acids (e.g. hydroxyproline)?

Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...

29 January 2021 8,300 4 View

What do you mean by 'excitation' of a plasma wave? What is the physical significance of this 'excitation'? Why is it necessary?

I have read numerous research papers on various mechanisms of excitation of surface plasma wave. But why do we need to excite a wave? Is it necessary? Can't the wave propagate without being excited?

25 January 2021 3,790 3 View

What is the difference between research summaries and abstracts? Can an abstract be contained in a research summary?

Some articles confuse the two subjects. I need to find out if they can be used interchangeably. If not, what is the difference between the two?

13 January 2021 847 3 View

How long does the peptide cleavage cocktail last before it gets expired?

The cleavage cocktail is composed of Phenol/thioanisole/water/TFA/triisopropylsilane. I only prepare 10 mL cocktail per peptide but can the activity of the cocktail prepared at 100 mL last for a week?

10 January 2021 7,424 3 View

How to present and discuss results, when methodology is not data-based?

Dear Everyone! I'd ask you opinion about a problem, which is returning in my "softer" research field. Time to time I work on research projects, which do not have available data and/or the data...

29 December 2020 7,637 6 View

Do anyone know a lab in Mexico where dielectric properties of solids and liquid can be measured?

I mean those kits that can be connected to vector network analyzers in order to find out permittivity and permeabilitty of solid and liquid from around 1 to 10 or 30 GHz

25 November 2020 852 1 View

How would you describe the most relevant research questions and directions in urban air pollution, if I'm specially interested in transportation?

I am relatively new to this field, my research interests are smart cities, shared mobility, transport service integration. We have data about CO, NO2, O3, PM10 from several cities. I would like to...

11 November 2020 9,099 4 View

How relevant is the Area under curve (AUC) in ROC being different from .50?

I analyze the psychometric qualities of a screening instrument to predict depression after stroke using ROC analysis. In this context, I read several papers which report an AUC without a p-value...

12 October 2020 6,557 4 View

How do you guys measure the rate of protein translation for a specific protein?

I am trying to think about a way to measure the protein translation rate for a single and specific protein that I am interested in. I've been thinking that Ribo-seq might be a good idea for this...

07 September 2020 9,054 3 View

Determination of Lipid A in Salmonella?

Im working with a S. typhimurium strain. Until now when i wanted to have a look at the amount of Lipid A present in the cells, i did silverstainings with proteinaseK treated whole cell samples....

26 August 2020 9,455 1 View

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01 March 2021 8,235 2 View

Mediation path analysis - Can I run two seperate models?

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27 February 2021 5,681 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

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SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

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Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

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21 February 2021 3,278 3 View

How to run repeated measures in mvabund?

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Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

Is it possible to conduct a simple linear regression with a continuous value as an DV and 3 categorical ones for the IV?

I am currently writing my dissertation on the potential effect of bilingualism on intelligence scores. The latter is represented by a scale on the Y axe. My independent variable is the linguistic...

24 January 2021 5,336 2 View

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