05 December 2017 1 796 Report

I am ideally looking for vignettes which describe dangerous crimes in a reasonable amount of detail, but without too much graphic detail, and where the evidence makes it unclear whether the defendant is guilty.

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How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

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SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

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How to use back-trajectory analysis (HYSPLIT) to assess air parcels at breathing/monitor height?

Hi there, I'm currently monitoring air pollution in Grenada, West Indies and as part of my project I have identified four scenarios in which I would like to use a back-trajectory analysis...

27 December 2020 730 4 View

Origin of the term "transposition" used in photovoltaic performance modeling?

In PV modeling, the process of elevating GHI data to a tilted surface is often referred to as "transposition". However, strictly speaking, "transposition" in mathematics refers to rearranging a...

22 September 2020 767 3 View

Help figuring out why only lifted cells express eGFP after transduction?

I'm attempting to transduce HEK293T cells with a lentivirus expressing eGFP. I have been observing a low transduction efficiency and after a 24 h transduction the only cells expressing eGFP are...

03 September 2020 9,491 5 View

How to stimulate PBMC-derived monocytes with TLR7 and TLR8 agonists?

I'm trying to stimulate human monocytes from fresh PBMC isolations with 2ug/mL imiquimod and 2ug/mL ssRNA40. I do this in 10% FCS complete RPMI for 18-20 hours before staining and analysing CD14+...

10 August 2020 7,095 1 View

NirS qPCR assay underestimating by 2 log?

My lab designed a qPCR assay for amplifying the denitrification gene nirS from DNA extracted from soil. The assay has worked perfectly in the past with almost perfect efficiency and gene copy...

20 July 2020 8,070 1 View

Why does Gaussian opt+freq job optimizes fine but crashes during Frec calc?

I am running jobs like # opt freq uwb97xd/6-311g(d,p) integral=grid=ultrafine geom=connectivity They complete the optimizations steps and then die during force calculations. An example of the...

08 July 2020 7,456 1 View

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