Hi, i don't know the answer to your question but i'm also trying to do an rda (i'm a beginner) but i don't know how you can tell what transformation you need to do. what kind of transformation did you do?

Aze Peeters Hi,

Well, there is a lot transformations that you can use for the data. For example, Hellinger´transformation is very recommended for abundance data. In my case, i used the standarization for environmental variables.

thanks A LOT, I will try this and see if i get the same error!

what function did you use? I standardized my environmental data and transformed my species data (just like you said) and did this

rda4

Now that I checked, that error appeared because the number of rows was not the same between the abundance data matrix and the environmental variables data matrix. You must make sure it is the same. For example, I have 38 species at 26 sites and 25 environmental variables at the same 26 sites and that's how it worked. The sites correspond to the rows.