How to calculate dilution factor based on the highest OD/concentration of sample in ELISA?

I am using Elabscience sandwich ELISA kit (detection range 0.31-20ng/ml), I am using supernatant saliva samples. I am using 4 plates and plotting the standard curve for every plate. In one of the...

27 January 2021 9,759 1 View

Error with atomic balancing in ASPEN PLUS ?

Hello, I am simulating MSW feed as a non-conventional using Aspen plus. In the RYIELD block I have entered the basis yield (mass basis). I am facing an error saying that ''THE following elements...

16 July 2020 8,188 4 View

I need a protocol for Cas9 expression in E coli?

Hello Dear researchers, I am trying to express Cas9 vector in E coli but finding difficulties. After IPTG induction I am unable to get any protein(Cas9) via His-tag chromatography. Also, there is...

17 June 2020 1,170 2 View

I want to give my master students research topics related to Text mining, which current trends regarding Natural language processing?

i want to give my master students research topics related to Text mining especially regarding language processing so anyone will guide me in this regard?

27 February 2020 8,526 4 View

How to identified fungal hyphae in fish muscle histology?

how to identified fungal hypahae in fish muscle histology?

20 March 2019 9,649 3 View

I want to apply hierarchical clustering on my corpus(text data) can any one tell me which algorithm will do this best ?

and please also tell me any software to use that algorithm?

02 November 2016 7,724 4 View

Can a power failure during the first spin of PBMC isolation ruin the buffy coat layer?

We have power trips going on quite frequently in our lab and to say the least this is a nightmare for my PBMC isolations. I was wondering if it would ruin the layer separation permanently or is...

07 February 2015 4,678 10 View

Hi, sir! Other than MoS2 suggest any other 2D materials that can be used as dye??

I am a research student....I want to discuss DSSC basic principle with varying dye to produce energy in order to overcome energy crisis....

01 January 1970 3,675 4 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View