How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Why below-ground ecology is still unexplored (majority of it) in our country ?

Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among...

02 January 2021 6,912 3 View

Can you give me some Information about the generation of ROS in marine surface water?

Reactive oxygen species is a very new term i think

23 December 2020 8,416 4 View

Anyone an expert in algae in Victoria, Australia?

I found this (very pretty) algae (see picture) in a bore-fed and suburban catchment run-off fed, human made lake (Newport Lakes) in Newport, Victoria, Australia. I'm a vertebrate zoologist with a...

09 November 2020 7,587 3 View

At what time points should pro-inflammatory cytokines levels be tested in a mouse model of Prototheca algae infection?

I'm working on establishing an murine infection model of the Prototheca algae. Not much is known about the dynamics of the infection, nor the induced immune response. I'd like to use the...

19 October 2020 2,908 2 View

Someone knows of a DNA purification protocol from polysaccharides (for brown seaweed extracts)?

Hi, I have this "bad" DNA extract of a Fucus spp. and I want to purifying this because the PCR analysis that I need don't show results. I alredy try to extract twice the DNA both with the Genomic...

06 October 2020 1,500 1 View

Which research institutes in India have an algal identification facility?

I am currently pursuing my research in Phycology and planning to conduct a study on microalgae. Is there any place where I can send my samples to be identified? (Preferably in South India) Also,...

17 September 2020 4,440 2 View

What is the best method for commercial production of Astaxanthin from Haematococcus pluvialis?

Dear Friends, I'm looking for a reference that has extensively explained the best method to increase the biomass of Haematococcus pluvialis algae as well as the extraction of Astaxanthin from it....

30 August 2020 2,920 3 View

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro (laboratory)culture?

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro culture? I am looking to replace the Provasoli medium with something simpler and cheaper (I have...

10 August 2020 302 3 View

How can the biological response to freshwater algae be used for environmental impact assessment?

What are the mathematical equations used to assess the environmental impact using some biological criteria in green algae?

17 July 2020 5,460 4 View