I have purified my target protein with an N-ter His-GST tag. Even after size exclusion, I see equivalent amount of free GST in the mix. I want to retain the tag for downstream assays. Is there any way of removing the free GST from my sample
I think you can use the Gel filtration (Sephadex) , as he notes in my research:
Article Partial Separation and Some Kinetic studies of Glutathione S...
Bhaba Krishna Das
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