This is a true branched cyanobacteria that we intend to identify. Anyone there who could give a clear idea of what those yellow colored large cells are?
Dear Shailesh,
I think those cells are heterocits, they have polar nodules.
Hello friends....I will add more pics....We have identified it as Westiellopsis, have sequenced it and done many other things....The problem is those large enlarged cells which forced me to ask this question here.
I just uploaded one plate. Hope it helps and hope it brings better understanding of this cyanobacteria.
Hi Prashant,
Yellow cells are definitely heterocytes. It seems that you have some pictures from older culture, therefore heterocytes are sometimes fuzzy and have a different shape. In the last figure (right picture in central row), there might be also a different species, which might also explain, why there are different shapes of heterocytes. On the other hand, I have noticed that sometimes Fisherella like cyanos change their morphology during culturing perhaps due to nutrient depletion.
Cheers and good luck!
Petr
This Symphyonemopsis angle is indeed interesting !!!!
The strain grows very well in culture. But I am thinking :)
We grew it in BG11 without nitrogen. The temperature and light is the routine ones. I personally do not like to play too much with these conditions unless and until there is some strain that needs to be tested for something. We handle a huge load of cyanobacteria and hence it will b4e realistically impossible for us to give special condition to every strain. I think 20-25 temperature is also fine. Sometimes, I do play with phosphorous for the akinetes, but rest of the things, I try not to disturb a winning combination.
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