Extraction of viral DNA from human blood?

Hi, I am looking to detect CMV DNA in immunocompromised leukemia patients followed by subsequent end-point PCR. Can anyone suggests a suitable kit for extraction of CMV DNA from human blood...

13 December 2020 4,024 5 View

Can the activity of IPTG induced protein affect bacterial growth?

I inserted 4 different genes in vector, and cloned in E.coli. when I grow them on IPTG containing plate, Each bacteria grow different. If I pick each bacteria with different number, then each...

26 March 2020 4,476 3 View

No title

29 October 2018 7,986 6 View

What do use any kind of an adhesive for BP - electrode in vanadium redox flow battery?

03 July 2016 3,866 1 View

What are problems due to the effect of backward facing step flow?

25 March 2016 4,575 1 View

The thickness of samples for FTIR analysis ATR mode should be thick or thin?

09 March 2016 5,144 5 View

What is the accepted measures for learning outcomes (i.e., the levels of learning outcomes for teams and individuals)?

not learning goal orientation nor learning behavior, but actual outcomes from learning

06 July 2015 2,012 3 View

Would it be possible to reliably detect eGFP expression level via qRT-PCR assay?

I would like to check the transcript copy of the GFP-tagged chimeric protein that I have introduced into the animal via viral vector via RT-qPCR. Although I can see the GFP in the histology of...

10 March 2015 4,874 3 View

What is the cutoff point for Intraclass correlation (ICC(1) and ICC(2)?

For a multilevel analysis, we need to aggregate a lower level variable to a higher level variable. What is the cutoff point for Intraclass correlation (ICC(1) and ICC(2)? Is there any agreement...

27 May 2014 7,877 17 View

What is the cutoff point for Intraclass correlation (ICC(1) and ICC(2)?

For a multilevel analysis, we need to aggregate a lower level variable to a higher level variable. What is the cutoff point for Intraclass correlation (ICC(1) and ICC(2)? Is there any agreement...

04 May 2014 4,300 12 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

No title

01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Which are the most recent implications of ESL Academic Discourse in language teaching?

Language teachers at UTP (Language Department) are increasingly interested in the role of academic papers in the development of language (English) teaching practices. Research papers in Academic...

22 February 2021 1,388 4 View