Hi there!
We are thinking about looking into the transmission modes of insect-specific viruses. One focus will be the potential infection of mosquito larvae via the water body in which they are developing. The current idea is to spike the rearing water with virus and then see, whether the larvae become infected.
Does anyone have experience in that sort of experimental set-up?
I'm grateful for any input/paper suggestions etc.
Thanks!
Hi, I work with Aedes aegypti and Ae. albopictus mosquitoes. In our experience the finding of infected larvae by dengue virus (by RT-PCR) in wild conditions is an event that ocurrers in low rates. The vertical transmission has been demostrated to explain these findings. Considering the posibility of ocurrence of infectious viral particles in water, I would think that the viral infection rates of larvae could be high. (In the most breeding sites the volume of water is low). However, It does not occur. Do you have empirical observations that support your hypothesis?
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