I have a blend of about 10 beneficial bacillus species for liquid fertilizer formulation. The supplier provided the list of species and their estimate numbers. The concentration of these species in the blend is not uniform. I would like to confirm the presence of these species and their quantity (i.e identification and enumeration). I have contacted more than half a dozen microbiological labs in North America, but none of them were able to do the test. Note that all of these labs advertised on their websites that they do conduct identification and enumeration.
Do you have the experience in this regard? or do you know any lab that is able to conduct the test?
Thanks,
Dereje
Hi Dereje
You mentioned that you have a list from supplier? do you mean that you know the species name? if so then search for test(s) that separate one from others for example if one them hydrolysed sucrose then use a medium that contained sucrose as the only carbon source in this medium, you need to test several substrates accordingly; frankly its not an easy, give it ago especially you know the names of the species, Bergey's Manuel will be useful for the species level identification, look for substrates that separate one from another in this Manuel.
You could use streak plate method for separating them as well where you ended up with a single colony of each then subculture it to have a pure culture check the following link
Ps I would go for streak plate first
http://faculty.sdmiramar.edu/dtrubovitz/micro/microhandouts/labs/pureculture.pdf
Hope this helps & good luck
Hello Dereje,
I think first of all you should separate the bacteria using streak plate method. after obtaining pure culture you should use Bergey's manuel for the biochemical identification after that you can indentify the species using 16SrRNA sequencing. You can use spectrophotometer for followed by most probable number method for the enumeration of bacteria.
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