How many tumor cells should I seed in a 96-well plate/well for virus infectivity?

The tumor cells are from a variety of cell lines from ATCC .

22 December 2020 1,950 3 View

How does band gap of a semiconductor shift with the lattice strain? and physical significance?

Someone posted a similar question but I have a further question on strain (increase/ decrease atom distance). So I know that the distance increases and decreases the bandgap distance. But can it...

04 June 2020 689 5 View

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance and yield attributing traits self pollinat?

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance in self pollinating crops (F2 generation) Vs cross pollinating crops, is it important to...

30 March 2020 1,011 3 View

What is the most appropriate statistical test for my experiment?

I am having trouble identifying the most appropriate statistical test for an experiment I am designing. In this experiment I am looking at multiple markers that indicate a change in structure...

25 November 2019 9,090 2 View

Where could I find some SOPs and formulations for making in house Clinical chemistry reagents?

Hi, I am looking for some resources to be able to find some SOPs and formulations for making in house Clinical enzymatic chemistry reagents like; Triglyceride, HDL-Cholestrol, Oxalate, Lactate,...

25 July 2019 698 2 View

Has anyone else had any problems with PSA antibodies for WB?

I am doing work on prostate cancer cell line - LNCaP - and using PSA antibody to show androgen deprivation. We currently have 3 in our lab, none of which are giving us a signal for western blots....

14 July 2019 2,723 3 View

How do you correct bond order in mol2 files for gromacs?

Hello, I am just learning to use gromacs following this tutorial: http://www.mdtutorials.com/gmx/complex/02_topology.html In the tutorial there is a script for rearranging the bond order of the...

16 January 2019 7,439 1 View

When thawing cryopreserved cells, can they be added to 0.9% NaCl without loss of viability?

The cells are cryopreserved in 8% DMSO and 2% HSA.

08 November 2018 3,807 3 View

What is best way to check for non-proliferation in a tumor cell lysate?

We use 6x freeze-thaw cycles and consistently show 0% viability by Trypan Blue. We have used MTT and results were good, but some say DNA assays are better. Thanks

03 August 2018 4,119 3 View

What is second order CFA?

Hi ? I ran CFA in AMOS and fit indices were poor . I ran EFA in SPSS (etracted six factors) and delete some Items that were cross-loading , then run CFA again and now fit indices are within...

30 July 2018 9,679 5 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

No title

01 March 2021 3,905 5 View

Which period is considered as study period in research?

Is that starting from proposal development to result writeup or just from data collection to result writeup and reporting (publishing)?

28 February 2021 910 2 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

Are we using now IT technology effectively?

I discover now two my papers, which are more then 30 years old and which are describing something, what was done in years 1985 -...

21 February 2021 5,008 3 View

Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View

Where I can find materials to learn Fuzzy VIKOR methodology?

Please suggest ways to learn Fuzzy VIKOR methodology in a best possible way. And is there any toolkit available for the same? Thank you in advance.

20 February 2021 8,271 3 View