I am planning to do a westernblot for the microglia marker Iba-1, using mouse brain extract . Please recommend a good antibody for westernblotting.
Anti-Iba1 antibody [1022-5] (ab15690)
https://www.abcam.com/iba1-antibody-1022-5-ab15690.html
In addition to the one suggested by Joaquim, the one form WAKO is also good
Link- http://www.wako-chem.co.jp/english/labchem/product/life/AntiIba1/index.htm
I have used them for mouse spinal cord and both work well
Thank you for the suggestions.
Hi Deepa Sathyaseelan ,
I'd recommend doing a search on BenchSci for published antibodies for your specific purpose (https://landing.benchsci.com/academic).
Please see attached for more info.
I hope this helps!
Maurice
Thanks, Maurice!
i already have output for TF*IDF output inpycharm...i required to plot a graph term and TF*IDF and document similarity and qurery comparision with documents. please suggest me using python code in...
29 May 2018 3,375 4 View
I am writing code for multi-carrier data transmission over time varying multi-path channel in MATLAB. Mathematically , received signal can be represented as, y=x(t)*h(t,delay)+n(t) Impulse...
20 May 2018 943 2 View
i have to measure protein concentration of bacteria on polyethylene surface. for this i used coomassie brilliant blue solution in perchloric acid as an indicator. but acetic anhydride is not...
18 February 2018 6,826 2 View
I have done in detail Raman studies for charge transfer between few layer MoS2 and donor and acceptor aromatic molcules (like TCNE, MV etc.).In general FWHM increase with increasing conc. of...
23 August 2017 9,529 2 View
I am trying to develop an ELISA to measure antibodies to Hepatits B surface antigen in human serum.My detection antibody is an anti-human IgG-HRP. I getting a proper sigmoidal standard...
27 July 2016 1,392 3 View
I used a protocol mentioned below and it did not work. dissolved single colony with 100ul of buffer(200mM LiAc,100mM SDS) incubated at 70C for 5min Added 300ul of icecold ethanol and vortexed...
14 March 2016 9,756 3 View
How can I differ optimal guaranteed Cost Control with optimal control? Yurong Liu, Department of Mathematics, China.
04 October 2015 1,904 5 View
I wanted to measure rat mast cell preotease-1 activity. Is there any ELISA kit available ? I couldnot find one. Also, if I have to make own plates and do it, what is the best antibody that have...
22 March 2015 3,086 2 View
I am trying to extract exosomes from mcf7 cell line. After the sequential centrifugation step I calculated the numberof exosomes per cell based on the nanosight data. It comes to be very low (of...
17 March 2015 1,932 9 View
19 January 2015 834 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View