I am trying to verify the binding of a polysaccharide to the mannose receptor, any recommendations on a possible antagonist that I can use ?
Many Thanks
You can either use the mAb clone 3.29B1 which blocks binding, or use dextran, and titrate this in in increasing concentrations. Problem might be that dextran will be internalised as well. You may also have issues finding a real antagonist as the MR recycles after being internalised, as well as new MR molecules showing up on the cellular surface. You could try using your molecule first, and see whether binding of FITC-dextran is reduced? Hope that helps a bit?
the Mannan is used regularly.
Antibodies: ERMP23, 122D2, 129C3 and 118F8 are also able to inhibit the mannose receptor CD206, but beware some antibodies can cause receptor internalization.
Is mannan not going to act as an agonist as it's composed of mannose ? Thanks
Mannan is working as an agonist for the receptor, but it will still comped with the binding of your molecule. It may depend what you use as read-out. Assessing inhibition of binding might be more successful compared to assessing some cellular responses.
is it true that Mannan does not only block MR but also others CLRs like DC-SIGN (CD209) ?
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