I've been growing my strain on Solar System 1000 light on full power. Also I've added a CO2 injection system recently to help with growing. I'm growing it in a Proteose peptone medium and adding an Airstone to help with Oxygen. Is there any advice one of you could give me? Cause it doesn't seem to be working.
@ Justin, algae growth is regulating by nutrient quantity and quality, light, pH, turbulence, salinity and temperature. Please look at the nutrient quantity and quality required by your species is enough in your media. Otherwise, as per Guillard and Ryther (1962) , F/2 is the perfect nutrient media for algae and a widely used general enriched medium for growing algae.
Initially make sure a very pure nutrient medium (Autoclaved water, sterilized flasks, culture area, air balls, and optimum light intensity). Make a balanced nutrient medium (F/2 or Zurroks) considering major and trace elements including vitamins. Initially, interval aeration is recommended. Once you can easily grow your culture when your stock will be healthy.